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BEGIN:VCALENDAR VERSION:2.0 METHOD:PUBLISH CALSCALE:GREGORIAN PRODID:-//WordPress - MECv6.5.5//EN X-ORIGINAL-URL:https://oursymposium.sites.unlv.edu/ X-WR-CALNAME:Office of Undergraduate Research (OUR) Fall 2021 Virtual Undergraduate Research Symposium X-WR-CALDESC: REFRESH-INTERVAL;VALUE=DURATION:PT1H X-PUBLISHED-TTL:PT1H X-MS-OLK-FORCEINSPECTOROPEN:TRUE BEGIN:VEVENT CLASS:PUBLIC DTSTART;TZID=UTC:20211115T000000 DTEND;TZID=UTC:20211120T000000 DTSTAMP:20211110T000000 UID:MEC-e22cb9d6bbb4c290a94e4fff4d68a831@oursymposium.sites.unlv.edu CREATED:20211110 LAST-MODIFIED:20211111 PRIORITY:5 TRANSP:OPAQUE SUMMARY:AHS-L2-2. Expression Patterns of Integral Axon Initial Segment Proteins During Postnatal Cortical Development DESCRIPTION:\n\nAmanjot Kailey1\nRachel Ali Rodriguez1\nJeffery Barker2\nDustin Hines, Ph.D.2\nFaculty Mentor: Rochelle Hines, Ph.D.2\n1College of Science, School of Life Sciences\n2College of Liberal Arts, Department of Psychology\nABSTRACT\nA key site in the control of neuronal activity patterns is a specialized compartment known as the axon initial segment (AIS). The AIS is a highly organized neuronal compartment whose local density of proteins and morphology in part determine the firing characteristics of neurons. Recent studies have demonstrated that the AIS undergoes important modifications during development, but the molecular mechanisms that affect the development, composition, and morphology of the AIS remain obscure.\nWe are using western blotting to examine the developmental expression of proteins that are enriched in the AIS across a time course of cortical development, focusing on the time points of postnatal day 5, 10, 20 and adulthood. Using antibodies we are able to specifically detect bands of proteins separated by molecular weight. We analyze these bands to determine intensity of signals a proxy for expression level. We normalize the intensity measured for each protein band to the abundant cytoskeletal protein actin, which allows us to control for the amount of sample loaded. We have found that the AIS protein Neurofascin undergoes a shift in abundance of specific isoforms during postnatal development, presenting an interesting target for further study. Understanding of the developmental regulation of AIS proteins has implications for understanding the development of cortical firing patterns and ultimately for disorders of cortical development.\n URL:https://oursymposium.sites.unlv.edu/program/expression-patterns-of-integral-axon-initial-segment-proteins-during-postnatal-cortical-development/ ORGANIZER;CN=The Office of Undergraduate Research:MAILTO:our@unlv.edu CATEGORIES:Arts, Humanities, and Social Sciences END:VEVENT END:VCALENDAR