AHS-L2-2. Expression Patterns of Integral Axon Initial Segment Proteins During Postnatal Cortical Development


Amanjot Kailey1
Rachel Ali Rodriguez1
Jeffery Barker2
Dustin Hines, Ph.D.2
Faculty Mentor: Rochelle Hines, Ph.D.2
1College of Science, School of Life Sciences
2College of Liberal Arts, Department of Psychology

ABSTRACT
A key site in the control of neuronal activity patterns is a specialized compartment known as the axon initial segment (AIS). The AIS is a highly organized neuronal compartment whose local density of proteins and morphology in part determine the firing characteristics of neurons. Recent studies have demonstrated that the AIS undergoes important modifications during development, but the molecular mechanisms that affect the development, composition, and morphology of the AIS remain obscure.
We are using western blotting to examine the developmental expression of proteins that are enriched in the AIS across a time course of cortical development, focusing on the time points of postnatal day 5, 10, 20 and adulthood. Using antibodies we are able to specifically detect bands of proteins separated by molecular weight. We analyze these bands to determine intensity of signals a proxy for expression level. We normalize the intensity measured for each protein band to the abundant cytoskeletal protein actin, which allows us to control for the amount of sample loaded. We have found that the AIS protein Neurofascin undergoes a shift in abundance of specific isoforms during postnatal development, presenting an interesting target for further study. Understanding of the developmental regulation of AIS proteins has implications for understanding the development of cortical firing patterns and ultimately for disorders of cortical development.

Date

Nov 15 - 19 2021
Expired!

Time

All Day

Labels

AHS: Lighting Talk Session 2
The Office of Undergraduate Research

Organizer

The Office of Undergraduate Research
Phone
702-895-4771
Email
our@unlv.edu
Website
http://unlv.edu/our

Speakers

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