HNSE-P1-5. Isolation of Salt Tolerant Bacteria and Investigation of Perchlorate Biodegradation at High Salinity Conditions

Aymen Shafique1
Yasaman Saedi2
Faculty Mentor: Jacimaria Ramos Batista, Ph.D.2
1College of Sciences, Department of Life Sciences
2Howard R. Hughes College of Engineering, Department of Civil and Environmental Engineering and Construction

Perchlorate (ClO4-), known as a highly soluble oxidizer contaminant, has been detected in soil and groundwater throughout the United States for the last two decades. Biological reduction has been seen as a promising technology through which perchlorate reducing bacteria (PRB) occurring naturally in most environments utilize ClO4- as an electron acceptor. However, biological reduction of perchlorate is influenced by high salinity levels because the PRBs’ activity is hindered at salinities over 1%. The goal of this study is to investigate the impact of salinity (NaCl: 0-6.5%) on perchlorate biological reduction. Actual soil samples from the contaminated site containing PRBs were used and acclimated in broths with different salt concentrations and later the cultures were tested on their reduction abilities using perchlorate (150 mg/L) as an electron acceptor (450 mg/L) and acetate as an electron donor. Gram-staining results revealed that there has been consistent growth of salt tolerant bacteria observed at each salt concentration level (0-6.5%). The variety of bacteria are displayed with the diverse shapes present under the compound microscope, from Gram-positive bacilli to Gram-negative cocci. However, it is important to consider that the reduction kinetic is slow even as the perchlorate reduction has been observed at 6.5% NaCl concentration. This will be further studied as the experiment is still running to obtain more promising results regarding perchlorate reduction and salt tolerant bacteria isolation. The conclusion drawn so far is that there are salt tolerant bacteria in this soil which can degrade perchlorate at environments with high salt concentration.


Nov 15 - 19 2021


All Day


HNSE: Poster Session 1
The Office of Undergraduate Research


The Office of Undergraduate Research


One Reply to “HNSE-P1-5. Isolation of Salt Tolerant Bacteria and Investigation of Perchlorate Biodegradation at High Salinity Conditions”

  1. Hello everybody! This is Aymen Shafique, the undergraduate researcher for this project. I would like to thank you for taking the time in viewing the work presented. If you have any questions regarding the content or would like to provide any feedback, you are more than welcome to do so in this here comment section. I’ll be checking it as often as I can to view and respond accordingly. Looking forward to hearing from you all soon!!

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