HNSE-P4-5. Regulation of Eye Stem Cells During Regeneration
Cindy X. Kha1
Faculty Mentor: Kelly Ai-Sun Tseng, Ph.D.1
1College of Science, School of Life Sciences
Regeneration is a response to injury that results in the regrowth of damaged or lost body parts. The mechanisms of this process are poorly understood. Studying a highly regenerative species can further our understanding of the natural regeneration process. The African clawed frog, Xenopus laevis, is an excellent model for studying regeneration as it can regenerate multiple structures including its eyes. Xenopus embryos regenerate eyes within 5 days (Kha et al., 2018). The Vacuolar-ATPase (V-ATPase) is a proton pump that moves protons across the plasma membrane. This pump is important in regulating membrane voltage and has been shown to be important for appendage regeneration. Chemical inhibition of V-ATPase blocked embryonic eye regeneration in Xenopus and resulted in a small regenerate eye. Thus, the function of V-ATPase is required for this process. This project aims to determine the role of V-ATPase during eye regeneration by examining the eye defects caused by V-ATPase inhibition. Insights to the regeneration process can lead to potential medicinal applications.
This research was funded by UNLV’s TRIO McNair Scholars Institute, which is housed within UNLV’s Center for Academic Enrichment and Outreach and funded under the TRIO Ronald E. McNair Postbaccalaureate Achievement Program by a grant (P217A170069) from the U.S. Department of Education.